What conclusions can draw about the effects of concentration, temperature and pH on the reaction rate after comparing the plots from the three different Procedures?
Procedure I – Enzyme Reaction Rate – Concentration Dependence
Complete the tables below using your data and information found under the Background tab (see the Summary of Needed Formulas section)
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| Trial | ConcentrationLevel | OxygenConcentrationChange Data (ppt) | ElapsedTime(s) | EnzymeReaction Rate(ppt/s) |
| 1 | 2.5% | 5.17 ppt | 20.0 | 0.2585 |
| 2 | 5.0% | 10.34 ppt | 20.0 | 0.517 |
| 3 | 10% | 20.68 ppt | 20.0 | 1.034 |
Observations and Questions
[1] Sample Calculation: Show your enzyme reaction rate calculation for Trial 1.
[2] Convert enzyme concentrations of 2.5%, 5.0%, and 10% to ppt units.
[3] For Procedure I/Table I, identify:
Independent variable: Oxygen Concentration
Dependent variable: Enzyme Reaction Rate
Control variables: Elapsed Time
[4] Draw a graph of the independent variable on the x-axis (abscissa) and the dependent variable on the y-axis (ordinate). Label the axis titles and units.
Procedure II – Enzyme Reaction Rate – Temperature Dependence
Complete the tables below using your data and information found under the Background tab (see the Summary of Needed Formulas section)
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| Trial | Temperature(C) | OxygenConcentrationChange Data (ppt) | ElapsedTime(s) | EnzymeReaction Rate(ppt/s) |
| 1 | 20.0 | 7.29 ppt | 20.0 | |
| 2 | 30.0 | 14.67 ppt | 20.0 | |
| 3 | 55.0 | 2.29 ppt | 20.0 |
Observations and Questions
[5] Sample Calculation: Show your enzyme reaction rate calculation for Trial 1.
[6] Using the data you reported in the Table above, describe the effect of temperature on the reaction rate.
Procedure III – Enzyme Reaction Rate – pH Dependence
Complete the table below using your data and information found under the Background tab (see the Summary of Needed Formulas section)
| Trial | pHLevel | OxygenConcentrationChange Data (ppt) | ElapsedTime(s) | EnzymeReaction Rate(ppt/s) |
| 1 | 6.0 | 6.28 ppt | 20.0 | |
| 2 | 7.0 | 10.34 ppt | 20.0 | |
| 3 | 9.0 | 1.40 ppt | 20.0 |
Observations and Questions
[7] Sample Calculation: Show your enzyme reaction rate calculation for Trial 1.
[8] Choose either a bar graph or a line graph format and plot the data for Procedures II and III. Plot the data for each Procedure on a separate plot.
[9] Compare the plot of the data for Procedures I, II, and III. Describe the differences among the three plots.
[10] What conclusions can draw about the effects of concentration, temperature and pH on the reaction rate after comparing the plots from the three different Procedures?
[11] Choose one of the factors that impacts reaction rate (concentration, temperature, or pH) and, in your own words, provide a plausible biological explanation for your experimental results for that factor.
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