How does the DNA polymerase “know” that it has incorporated an incorrect base?
Chapter 24
What are the functions of the three bacterial translation initiation factors?
What are the roles of bacterial elongation factors EF-Tu and EF-Ts?
What catalytic molecule of the ribosome is responsible for formation of the peptide bond?
What is the role of elongation factor EF-G in bacterial translation?
What are the paths of tRNAs and the mRNA through the bacterial ribosome?
What are the many parts that make up an mRNA molecule?
Chapter 25
How does degeneracy of the genetic code minimize the effects of mutations?
What is the wobble hypothesis, and how is it consistent with the third-base degeneracy of the genetic code?
What features of a tRNA allow its unique recognition by an aminoacyl-tRNA synthetase?
What are the differences between the tRNA-protein interactions of class I and class II synthetases?
How is the error rate of an aminoacyl-tRNA synthetase minimized?
Chapter 26
In a negative inducible gene system, what would be the effect of a mutation that eliminates the regulator?
In a positive inducible gene system, what would be the effect of a mutation that eliminates the regulator?
In the lac operon, what would be the effect of a mutation in the operator?
Why does the lac operon need three operators?
How does a hairpin structure in the mRNA cause transcription termination on the DNA?
Chapter 13
Why would a DNA polymerase enzyme need to have nuclease activity?
How does the DNA polymerase “know” that it has incorporated an incorrect base?
Why can’t the DNA polymerase move continuously on both strands?
Since SSB does not have an enzymatic function, why is it essential for replication?
Why couldn’t the transcription RNA polymerase be used to synthesize the primer?